|
KMID : 1140120080130020096
|
|
Cancer Prevention Research
2008 Volume.13 No. 2 p.96 ~ p.101
|
|
|
A Underlying Mechanism of IL-3-dependent GATA-3-mediated Bone Marrow-derived Mast Cell Proliferation
|
|
Park Cheung-Seog
Ahn Hyun-Jong
|
|
|
Abstract
|
|
|
|
Mastocytoma is one of cutaneous mastocytosis which is a hematopoietic neoplasm characterized by excessive proliferation of mast cells. Mature mast cells have the capacity to mediate both innate and Th2-mediated immune responses. The mechanisms regulating mast cell function in vivo have largely focused on cytokines, such as stem cell factor, IL-3, IL-4 and IL-10. Despite these advances, little attention has been paid to mechanisms that proliferate mast cells. Here we report effect of GATA-3 on the proliferation of murine bone marrow-derived mast cells (BMMC) by IL-3. BMMCs are generated from unseparated bone marrow in IL-3 containing medium. GATA-3 was induced in normal BMMC, when stimulated with IL-3. GATA-3 or dominant negative GATA-3 (dGATA-3) coding expression vector was trnasfected to BMMC. These BMMCs were recovered in IL-3 containing medium. Cells were restimulated with IL-3 after starvation. BMMC-dGATA-3 exhibited low levels of proliferation contrast to BMMC-GATA-3. In addition, there were no effect on the proliferation by culture supernatant of BMMC-GATA-3 and neutralizing anti-IL-4 Ab did not block GATA-3 induction by IL-3. These results indicate that the induction of GATA-3 without autocrine cytokine induction by IL-3 represents one of the mechanisms underlying mast cell proliferation. (Cancer Prev Res 13, 96-101, 2008)
|
|
|
KEYWORD
|
|
|
Mastocytoma, GATA-3, Mast cell, Proliferation
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|